By Erica Spackman (eds.)
Avian Influenza Virus, moment Edition goals to supply the basic tools utilized in operating with animal influenza viruses, and to collect extra complex details that would consultant the person in designing influenza reviews. Influenza A viruses are one of the most vital pathogens for people, nutrition animals and better half animals. learn and diagnostics with animal influenza viruses are serious to animal future health and it's going to be well-known that the desires and targets of animal agriculture and veterinary drugs usually are not continually almost like these of public overall healthiness. This quantity varieties out the diversities within the constitution of the bird, swine and equine industries, the organic modifications of influenza virus from each one animal workforce, and gives host, pressure and lineage particular suggestions and systems. Written within the winning Methods in Molecular Biology sequence structure, chapters contain introductions to their respective issues, lists of the required fabrics and reagents, step by step, comfortably reproducible protocols, and notes on troubleshooting and keeping off identified pitfalls.
Authoritative and obtainable, Avian Influenza Virus, moment Edition seeks to serve either execs and rookies with its well-honed methodologies for you to additional our wisdom of those vital pathogens.
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Additional resources for Animal Influenza Virus
14. dNTPs. 15. Glycerol. 16. Cryo-vials. 3 Cell Culture and Transfection 1. Cells: 293T (human embryonic renal epithelial cell line into which the gene for the temperature-sensitive Simian virus 40 T antigen has been inserted), Vero (African green monkey kidney), and Madin-Darby canine kidney (MDCK) cells can be obtained from ATCC (Manassas, VA). Primary chicken embryo fibroblast (CEF) cells are prepared from 10-day-old embryonating chicken eggs. 2. Dulbecco’s Modified Eagle’s Medium (DMEM). 3.
Pdf. Accessed 1 June 2013 Swayne DE, Senne DA, Beard CW (1998) Avian influenza. In: Dufour-Zavala L (ed) A laboratory manual for the isolation and identification of avian pathogens, 4th edn. American Association of Avian Pathologists, Kennett Square, PA, pp 128–134 Chapter 3 Neuraminidase-Inhibition Assay for the Identification of Influenza A Virus Neuraminidase Virus Subtype or Neuraminidase Antibody Specificity Janice C. Pedersen Abstract The neuraminidase-inhibition (NI) assay is a laboratory procedure for the identification of the neuraminidase (NA) glycoprotein subtype in influenza viruses or the NA subtype specificity of antibodies to influenza virus.
Biochem J 81:384 3. Palmer DF, Coleman MT, Dowdle WR, Schild GC (1975) Advanced laboratory techniques for influenza diagnosis. Immunology series No. 6. US Department of health, education and welfare, Washington, DC pp 18–22 4. Van Deusen R, Hinshaw V, Senne D, Pellacani D (1983) Micro neuraminidase-inhibitions assay for classification of influenza A virus neuraminidase. Avian Dis 27:745–750 5. Swayne DE, Halvorson DA (2003) Influenza. In: Saif YM, Barnes HJ, Gilsson JR, Fadly AM, McDougald LR, Swayne DE (eds) Diseases of poultry, 11th edn.
Animal Influenza Virus by Erica Spackman (eds.)